Select Sequencing Primers:   Custom primers

Custom Primers
We recommend that all sequencing primers be designed with an optimal length of 18-26 bases, a GC content of 50-60% and an annealing temperature between 50-65 o C.   Avoid sequences containing more than three identical contiguous bases.  

Primer concentration & volume required/well :

Submission format
All templates & primers should be submitted in 96-well, skirted PCR reaction plates (127.75mm x 85.65mm x 15mm; Island Scientific, cat# IS 800; MJ Research, cat# MSP 9601; Fisher Scientific, cat# 05-500-68 or VWR, cat# 53503-283).   Submit DNA and primers in separate plates, making sure that they are arranged in the identical corresponding positions.   (For example, template DNA for sample 8 should be loaded in well 8 of the Template Plate and its sequencing primer is loaded in well 8 of the Primer Plate). Partially filled plates will be charged as full plates .

Load primers in the following format :